No Audio on Start up - Resets to 0

Hello @whintyr, welcome to the forums.

Have you tried manually setting the settings by going to this location Documents\Klei\DoNotStarveTogether OR Documents\Klei\DoNotStarveTogetherANewReignBeta (if you're playing on the anewreignbeta branch) and right click client.ini and edit with notepad, at the bottom of the text would be the audio settings. Try tweaking those and then start up DST again, but be sure to have DST closed before you edit the file.

Cheers.

In addition to what Zillvr has provided, please ensure that your audio drivers have been updated to the latest version. Out-of-date drivers may cause issues with various software.

Also, it may also be that any change to your option settings are not saved due to your Windows User Account not having the proper administrative permissions. Here's how to check: https://www.cnet.com/how-to/how-to-check-if-your-windows-10-account-is-administrator/