After loading the game, my lower pneumatic doors to my hamster generator room refused access to my duplicants that have access to the "default" direction setting.
I am unsure the exact cause but its probably a bug when it saves if you load the bugged doors, it will remain bugged in future doors at that location during future saves unless you reset the default settings or decontruct the door.
I loaded the same game save 3 times and it refused access all 3 times so what ever the problem is, its in the save.
Its possible to regain access by disabling and re-enabling the default directions or giving the specific duplicant their own access to the room (but this doesn't fix the defaults error if you turn off the duplicant specific direction)
The error is in the save process or on loading the game. (save file enclosed)