I have shine bug critters that I enclose with some pneumatic doors, this has always worked well.
last night I started over on a new preview map, and they've started to sneak through doors when they open... and at least in the past Pneumatic Doors were supposed to work as a way to keep critters in place.
I open the doors to let them through, close it when they are in place, I look away for a moment, and suddenly they are in the same pen again >_<
Divide shine bugs along 2-3 adjacent rooms, with pneumatic doors. Keep doors on auto, wait and watch.