Spider Queen nests

not sure what you mean, but I think one of this will anwer it?

- spiderqueen will spawn spiders

- if you kill the spiderqueen, you will get spideregg:
http://dontstarve.wikia.com/wiki/Spider_Queen

Edited September 3, 2016 by Serpens

i think he means sometimes new spider den won't spawn when spider queen emerges.

Read the wiki for spider queen. A possible conditon is if there are more than 4 spider dens+spider queens nearby, spider queen won't spawn a new spider den.

If you wanna reproduce spider den you'd better only put 2 together, after a season they would yield 2 queens with 2 new spider dens.

Theoretically, if you put 3 together, they would yeild 3 queens with only 1 new spider den.

Edited September 23, 2016 by Amberboss